Development and validation of an enzyme-linked immunosorbent assay for antibodies against Mycobacterium bovis in european wild boar

Development and validation of an enzyme-linked immunosorbent assay for antibodies against Mycobacterium bovis in european wild boar

Blog Article

Abstract Background Bovine tuberculosis (bTB) Bosch PPQ7A6B90 Serie 6 75cm Tempered Glass 5 Burner Gass Hob - Black remains a significant problem in some parts of Spain largely because of contacts between cattle and wildlife reservoirs in extensive grazing systems.European Wild boar (Sus scrofa) is one of the species involved in the transmission of the disease to other species.Fast and simple detection methods would be critical for assessing infection prevalence, study the mechanisms of pathogen transmission and monitoring the effects of TB control measures.

Results An enzyme-linked immunosorbent assay (ELISA) to detect antibodies against Mycobacterium bovis in wild boar serum was developed and validated on 185 sera from TB positive and negative wild boar.Based on antigen inoculation of captive animals as Stirrup Irons well as tuberculosis compatible lesions, culture results and molecular analysis of hunted individuals, animals were allocated into two groups: tuberculosis positive group and tuberculosis negative group.After optimization of the positive to negative ratio using different combinations of serum dilutions and conjugate concentrations, the test yielded a sensitivity of 72.

60% and a specificity of 96.43% for the best cut-off.Conclusion Although some negative group animals showed an ELISA positive reaction (.